CSP Labs Blog » 2014 » December

CGMMV Testing by Seed ELISA and qRT-PCR

Sukhi Pannu - Director, Testing Services

December 10th, 2014
Sukhi Pannu - Director, Testing Services

Cucumber Green Mottle Mosaic Virus (CGMMV), a quarantine pathogen that infects a number of cucurbit crops, has been found in the U.S. and Australia over the last two years. In an effort to help control the occurrence of the disease the American Seed Trade Association (ASTA) has published a brochure in which it’s recommended that seed lots be tested by the National Seed Health System (NSHS) approved and International Seed Testing Association (ISTA) validated method. The method is an enzyme linked immunosorbent assay (ELISA) in which 1,500-2,000 seeds, or 10% of the total for smaller lots, are tested. Australia’s Department of Agriculture, Fisheries and Forestry (DAFF) is requiring that 9,400 seeds are tested by ELISA, and may soon require that a real-time RT-PCR (qRT-PCR) method is used.

You can find more information regarding causes, diagnosis, and control of CGMMV in the ASTA CGMMV brochure.

CSP Labs continues to run seed ELISA and qRT-PCR assays, both in a high-throughput fashion, capable of fast turnaround even for the large sample sizes (9,400 seeds) as required by DAFF.

Please contact us regarding your testing needs by calling or emailing Sukhi Pannu at (916) 655-1581 or